Coding

Part:BBa_K2926055:Design

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


Fusion protein of Flo11 from yeast, mCherry and pVIII from the M13 bacteriophage


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable proper protein folding we inserted short glycine-serine linkers between every subdomain.


Source

mCherry was obtained from the iGEM part BBa_J06504, the M13 bacteriophage protein pVIII was excised from a helperphage plasmid purchased from NEB and the n-terminal domain of the yeasts protein Flo11 was obtained through gene synthesis.

References